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KMID : 0363420180320010019
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Journal of Korean Oriental Pediatrics
2018 Volume.32 No. 1 p.19 ~ p.29
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The Anti-inflammatory Effect of Cheongyeonsan for Allergic Rhinitis
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Kim Jeong-Won
Ahn Sang-Hyun
Kim Ki-Bong
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Abstract
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Objectives: This study investigated the effects of Cheongyeonsan for allergic rhinitis.
Methods: First, the GS/MS was used to analyze the effects of Cheongyeonsan by measuring inflammatory markers. Second, 3 groups of 10 6-week-old BALB/c mice were divided into Ctrl (no treatment), ARE (allergic rhinitis-induced without treatment), and CRT (allergic rhinitis-induced after Cheongyeonsan treatment) groups. Ovalbumin (OVA) was used as an antigen to induce allergic rhinitis and sensitization was performed by intraperitoneal injection of 0.1% OVA solution 21, 14, and 7 days before the onset of allergic rhinitis. Allergic rhinitis was induced by dropping OVA solution on the nasal cavity of each mouse for 5 days after the last sensitization. Seven days after the first induction, second induction was introduced by the same method. After making the section, MMP-9, substance P, TNF-α, NF-κB p65, COX-2, iNOS and Nrf, apoptotic cells were observed by Masson trichrome staining, immunohistochemical staining, TUNEL of nasal mucosal tissues of each group.
Results: GC/MS results showed undecanoic acid. Masson trichrome results showed that the CRT group had less respiratory epithelial damage. Immunohistochemical staining showed CRT group had 58% decrease in MMP-9, 61% decrease in substance P, 55% decrease in TNF-α, 38% decrease in NF-κB p65, 53% decrease in COX-2, 54% decrease in
iNOS, 87% increase in Nrf compared to those of the ARE group. TUNEL showed a positive reaction of 84% increase in apoptotic cells greater than that of the ARE group.
Conclusions: Cheongyeonsan alleviates nasal mucosal damage and reduces inflammatory mediators from allergic rhinitis-induced mice.
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KEYWORD
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Cheongyeonsan, Allergic rhinitis, Substance P, TNF-α, NF-κB p65
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